epMotion® 5075t NGS solution

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epMotion® 5075t NGS solution
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epMotion® 5075t NGS solution
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epMotion® 5075t NGS solution
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epMotion® 5075t NGS solution
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View through epMotion workdeck with TM 300 tool with X50 and Illumina sequencer in background with lab person
Promotion
epMotion® 5075t NGS solution
Promotion
epMotion® 5075t NGS solution
Promotion
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
View through epMotion workdeck with TM 300 tool with X50 and Illumina sequencer in background with lab person
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
epMotion® 5075t NGS solution
play epMotion® 5075t NGS solution
play epMotion® 5075t NGS solution
play epMotion® 5075t NGS solution
play epMotion® 5075t NGS solution
. epMotion® 5075t NGS solution

NGS Made Easy - The epMotion 5075 NGS solution combines automated liquid handling and unique software features with the accessories and high-quality consumables you need for reliable NGS library preparation and maximum NGS kit flexibility. Next-generation sequencing sample preparation is a labor-intensive process, which requires experience, precision and accuracy to generate high-quality NGS libraries. Automating NGS library preparation eliminates the risk of human pipetting errors, provides reproducible results and increases your productivity. A vast list of automated NGS sample prep methods has been qualified by kit manufacturers and customers to deliver sequencing results that are comparable or better to those from manual preparation. In addition, an intuitive software interface allows for ease-of-use and rapid method design, while providing the needed flexibility to optimize even the most complex NGS workflows. More information

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Product Information

The epMotion 5075 NGS solution includes everything to start automated library preparation. The basic system is an epMotion 5075t with an integrated Eppendorf ThermoMixer® to mix, heat or cool samples and reagents, and a thermal module on deck position C2 for additional temperature control. A gripper for labware transport and 2x single- and 2x 8-channel pipetting tools to cover a volume range from 1 to 300 µL. NGS specific accessories (Thermoblock 96 OC, 2 x Thermoadapter 96-well, Rack ILMN tubes, ReservoirRack, Eppendorf Magnet Adapter FLX, waste bag holder) are included, plus NGS specific consumables (Eppendorf twin.tec® LoBind PCR Plate 96 semi-skirted, epT.I.P.S.® Motion pipette tips with filter PCR clean 50 µL & 300 µL each 960 tips, LiquidWasteTub 400 mL, 30 mL reservoirs, waste bags). The epMotion 5075 NGS solution is available from stock with and without CleanCap option in all different voltage versions.

ThermoMixer®


Fully integrated ThermoMixer with 2DMix-Control improves yield and application results, e.g. resuspending bacteria pellets or magnetic beads. Peltier element for heating and cooling of sample and/or reagents. The following epMotion versions are equipped with ThermoMixer capability, M5073, 5075t, 5075m, 5075vt. A ThermoMixer upgrade product is available for epMotion 5075l, 5075v, and successor versions 5075LH and 5075VAC.

epMotion® 5075t NGS solution

Sample preparation for Next Generation Sequencing (NGS)


involves extensive manual processing that can be subject to human error. Eppendorf epMotion Liquid Handling systems are important tools for labs that want to significantly increase throughput and consistency of results. The automation of labor-intensive steps in NGS sample prep, like pipetting, mixing, temperature control and magnetic separation brings about a standardized and streamlined process, with significantly reduced hands-on time and minimization of human errors.

epMotion® 5075t NGS solution

Gripper Tool


The gripper tool allows transport of labware on the worktable of epMotion 5073 or 5075 versions. It is loaded by automatic tool exchange and can be programmed easily in the software. The stacking of up to 5 plates is possible.

epMotion® 5075t NGS solution

Features

  • Same as 5075t, plus
  • Industry-proven, large repertoire of manufacturer and customer qualified NGS methods guarantees sequencing results comparable or better to those from manual preparation
  • Intelligent Tool Selection automatically decides which tools (single or 8-channel) to use for fastest run time – ideal when sample numbers change frequently or are not a multiple-of-8
  • Optimized bead clean-up on magnetic plate adapter to prevent carryover contamination
  • Email notification option keeps you informed about the status of your library prep run
  • Increased stacking capabilities for plates and tip boxes for more efficient use of deck space
  • Integrated, high-performance ThermoMixer and thermal module allow for efficient mixing and reliable temperature control
  • CleanCap option provides UV decontamination and HEPA air filter to protection samples from contaminations
  • Automating NGS library preparation using epMotion provides reproducible results due to its outstanding pipetting accuracy (0.31% at 1 µL) and precision (1.97 % at 1 µL) and by eliminating the risk of human pipetting errors

Applications

  • Like 5075l, plus
  • Bead applications with mixing and tempering
  • Sequencing and PCR clean-up
  • Nucleic acid purification
  • Cell disruption
  • Immunoassays
  • NGS library preparation
 epMotion® 5075t NGS solutionepMotion® 5075t NGS solutionepMotion® 5075t NGS solutionepMotion® 5075tC NGS solution
Gripper carrying capacity1,200 g1,200 g1,200 g1,200 g
Mixing frequencyOff, 300 – 2,000 rpm, optimized for labware and applicationOff, 300 – 2,000 rpm, optimized for labware and applicationOff, 300 – 2,000 rpm, optimized for labware and applicationOff, 300 – 2,000 rpm, optimized for labware and application
Temperature homogeneity≤ 10 % on the block≤ 10 % on the block≤ 10 % on the block≤ 10 % on the block
Heating rate5 °C/min, on the block5 °C/min, on the block5 °C/min, on the block5 °C/min, on the block
Cooling rate3 °C/min, on the block, above RT3 °C/min, on the block, above RT3 °C/min, on the block, above RT3 °C/min, on the block, above RT
Power supply100 – 240 V ±10 %, 50 – 60 Hz ±5 %100 – 240 V ±10 %, 50 – 60 Hz ±5 %100 – 240 V ±10 %, 50 – 60 Hz ±5 %100 – 240 V ±10 %, 50 – 60 Hz ±5 %
Max. power consumption700 W700 W700 W700 W
Dimensions (W × D × H)107 × 61 × 67 cm / 43 × 24 × 27 in107 × 61 × 67 cm / 43 × 24 × 27 in107 × 61 × 67 cm / 43 × 24 × 27 in107 × 61 × 77 cm / 43 × 24 × 30 in
Temperature range-15 °C below RT to 95 °C measured in labware at 75 % filling height-15 °C below RT to 95 °C measured in labware at 75 % filling height-15 °C below RT to 95 °C measured in labware at 75 % filling height-15 °C below RT to 95 °C measured in labware at 75 % filling height
Conductor – X, Y, Z Positioning    
Random measurement error±0.1 mm±0.1 mm±0.1 mm±0.1 mm
Systematic measurement error±0.3 mm±0.3 mm±0.3 mm±0.3 mm
Detector    
Optical confocal infrared detectorContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantities
Optical sensorContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantitiesContact-free recognition of fill level, inserted tools, labware, tip types and quantities
Dispensing tool    
Random measurement error (1 µL)1,2≤3 %≤3 %≤3 %≤3 %
Random measurement error (1,000 µL)1,2≤0.15 %≤0.15 %≤0.15 %≤0.15 %
Random measurement error (50 µL)1,2≤0.4 %≤0.4 %≤0.4 %≤0.4 %
Systematic measurement error (1 µL)1,2±5 %±5 %±5 %±5 %
Systematic measurement error (1,000 µL)1,2±0.7 %±0.7 %±0.7 %±0.7 %
Systematic measurement error (50 µL)1,2±1.2 %±1.2 %±1.2 %±1.2 %
1in pipetting mode, free jet, without pre-wetting, with distilled water, at 20 °C
2See Eppendorf Application Note 168 for typical pipetting performance