DASbox® Mini Bioreactor System

DASbox® Mini Bioreactor System
DASbox® Mini Bioreactor System
DASbox® Mini Bioreactor System
DASbox® Mini Bioreactor System
play DASbox® Mini Bioreactor System

With small working volumes, compact design and advanced control and information management options the Eppendorf DASbox Mini Bioreactor System addresses demands in research and early process development. Combined with the DASware Software Suite it is an optimum tool for Design of Experiments (DoE) approaches as well as standardising and streamlining process development. More information

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Product Information

The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-free temperature control and exhaust condensation satisfy users with easy handling.
In addition to using industry standard glass bioreactors the DASbox can be equipped with Eppendorf BioBLU 0.3 vessels, all fully instrumented single-use mini bioreactors.

Features

  • Parallel set-up of up to 24 bioreactors
  • Excellent scalability and reproducibility in both microbial and cell culture applications
  • Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as BioBLU 0.3 Single-Use Vessels
  • Small working volumes save on the amount of cell material, media and supplements required
  • Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel
  • Individual temperature control with liquid-free heating and cooling (Peltier)
  • Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode
  • LC display with key process parameters and integrated alarm function simplifies monitoring
  • Fully mass flow-controlled gas mixing with individual gas mixture from air, N2, O2, and CO2, each directable either to headspace or sparger
  • Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available
  • Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h
  • Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable
  • Optional pull-out system for enhanced accessibility of bioreactors and control unit

Applications

  • Process development in cell culture and microbiology
  • Controlled cultivation of stem cells
  • Design of Experiments (DoE)
  • Media optimization
  • Clone and cell line screening, strain characterization
 DASbox® Cell CultureDASbox® Cell Culture Single-UseDASbox® MicrobiologyDASbox® Microbiology Single-Use
ApplicationCell culture2Cell culture2MicrobiologyMicrobiology
Number of parallel unitsUp to 24Up to 24Up to 24Up to 24
SoftwareDASware control, other DASware optionalDASware control, other DASware optionalDASware control, other DASware optionalDASware control, other DASware optional
User InterfaceProcess computer with monitorProcess computer with monitorProcess computer with monitorProcess computer with monitor
Typical power consumption (4-fold system without process computer)168 W (at 230 V)/
154 W (at 115 V)
168 W (at 230 V)/
154 W (at 115 V)
168 W (at 230 V)/
154 W (at 115 V)
168 W (at 230 V)/
154 W (at 115 V)
Dimensions (W × D × H, 4-fold system without process computer)30 x 70 x 49 cm
(12 x 28 x 19 in)
30 x 70 x 49 cm
(12 x 28 x 19 in)
30 x 70 x 49 cm
(12 x 28 x 19 in)
30 x 70 x 49 cm
(12 x 28 x 19 in)
Typical weight (4-fold system without accessories)44 kg44 kg44 kg44 kg
Bioreactors    
VesselsGlass vesselsSingle-use vesselsGlass vesselsSingle-use vessels
SterilizationAutoclavablePre-sterilizedAutoclavablePre-sterilized
Total volume350 mL380 mL350 mL380 mL
Agitation    
DriveDirect overhead drivesMagnetic overhead drivesDirect overhead drivesMagnetic overhead drives
Speed ranges20 – 2500 rpm20 – 500 rpm20 – 2500 rpm20 – 2000 rpm
ImpellersMarinePitched-bladeRushton-typeRushton-type
Gassing    
Gas supplyTMFC; overlay and/or spargerTMFC; overlay and/or spargerTMFC; spargerTMFC; sparger
Standard gas flow rates0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO20.04 – 5 sL/h, 0.04 – 3.5 sL/h CO20.2 – 25 sL/h, 0.2 – 18 sL/h CO20.2 – 25 sL/h, 0.2 – 18 sL/h CO2
Standard gas mixingAir, N2, O2 and/or CO2Air, N2, O2 and/or CO2Air, N2, O2 and/or CO2Air, N2, O2 and/or CO2
Feeding    
Feed lines per vessel2 (standard)/
4 (optional)
2 (standard)/
4 (optional)
2 (standard)/
4 (optional)
2 (standard)/
4 (optional)
Standard feed rates (depending on tube diameter)0.3 – 9.5 mL/h to 13 – 420 mL/h0.3 – 9.5 mL/h to 13 – 420 mL/h0.3 – 9.5 mL/h to 13 – 420 mL/h0.3 – 9.5 mL/h to 13 – 420 mL/h
Monitoring and Control    
Temperature controlLiquid-free heating and cooling (Peltier)Liquid-free heating and cooling (Peltier)Liquid-free heating and cooling (Peltier)Liquid-free heating and cooling (Peltier)
Standard temperature range (at 25° C room temp.)10 – 60°C10 – 45°C10 – 60°C10 – 45°C
pH controlCO2/base, and other set-upsCO2/base, and other set-upsAcid and/or base, and other set-upsAcid and/or base, and other set-ups
DO controlCascade (O2 concentration, gas flow rate), and other set-upsCascade (O2 concentration, gas flow rate), and other set-upsCascade (agitation speed, O2 concentration, gas flow rate), and other set-upsCascade (agitation speed, O2 concentration, gas flow rate), and other set-ups
ORP (redox) measurement--Optional (select redox or level)Optional (select redox or level)
Level/foamOptionalOptionalOptional (select redox or level)Optional (select redox or level)
OD measurementOptional
(DASGIP OD4)
Optional
(DASGIP OD4)
Optional
(DASGIP OD4)
Optional
(DASGIP OD4)
Exhaust condensationLiquid-free (Peltier)Liquid-free (Peltier)Liquid-free (Peltier)Liquid-free (Peltier)
Exhaust analysis--Optional
(DASGIP GA4)
Optional
(DASGIP GA4)