BioBLU® c Single-Use Vessels

BioBLU® c Single-Use Vessels
BioBLU® c Single-Use Vessels
BioBLU® c Single-Use Vessels
BioBLU® c Single-Use Vessels
BioBLU® c Single-Use Vessels
BioBLU® c Single-Use Vessels
play BioBLU® c Single-Use Vessels
. BioBLU® c Single-Use Vessels

Made from monolayer virgin plastics the Eppendorf BioBLU Single–Use Vessels address the widely discussed problems associated with leachables and extractables (L&E), helping to make laboratories more efficient and safe. The rigid wall stirred–tank design mimicks traditional vessels and makes scale-up easy. More information

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Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing. BioBLU c and p vessels cover a wide variety of processes, including shear-sensitive suspension cell cultures and adherent cell line development.

Features

  • Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 100 mL to 40 L working volume
  • All BioBLU c vessel sizes available with pitched-blade impellers for cell culture applications
  • BioBLU 5p vessels with Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel® Disks
  • Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
  • Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
  • Raw materials used to produce BioBLU vessels comply with USP Class VI standards and are BSE/TSE free
  • Vessels are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
  • For use with Eppendorf benchtop bioreactor systems
  • Adaptor kits for legacy bioreactor controllers available
  • Validation packages available upon request

Applications

  • Insect, mammalian, and human suspension cell lines
  • Stem cells
  • Adherent cells
 BioBLU® 14cBioBLU® 0.3cBioBLU® 3cBioBLU® 1cBioBLU® 5pBioBLU® 50c
Validatableyes
Standard set-upBenchtop/Third-party
Bioreactors      
SterilizationPre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)
Working volume (total)3.5 – 10.5 L (14 L)100 – 250 mL (380 mL)1.25 – 3.75 L (5 L)320 mL – 1.25 L (1.8 L)13.75 L (5 L)18 – 40 L (50 L)
Material vessel3PS, PC (USP Class VI)PS, PC (USP Class VI)PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)
Material tubingSilicone, C-FlexSilicone, C-Flex2Silicone, C-FlexSilicone, C-Flex2Silicone, C-FlexSilicone, C-Flex
Head plate ports1x Pg 13.5, 1x harbest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 1x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust
Agitation      
DriveMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drives
ImpellersPitched-bladePitched-bladePitched-bladePitched-bladePacked-bedPitched-blade
Recommended agitation speed25 – 200 rpm20 – 500 rpm25 – 200 rpm30 – 600 rpm25 – 200 rpm25 – 150 rpm
Gassing      
Gas supplySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlay
Monitoring and Control      
Sensor length120 mm225 mm220 mm
Temperature sensorPt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)
Max. operating temperature40 °C40 °C40 °C40 °C40 °C40 °C
pH sensorOptical pH or standard glass sensorOptical pH or standard glass sensor (120 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (120 mm)Optical pH or standard glass sensor (625 mm)
DO SensorPolarographic DO - 12/355 mmPolarographic DO - 4.7/118 mmPolarographic or optical DO - 12/225 mmPolarographic DO - 4.7/229 mmPolarographic DO - 12/120 mmPolarographic DO - 12/526 mm
Exhaust condensationElectric heater bandLiquid-free cooling (Peltier)Electric heater band or PeltierLiquid-free cooling (Peltier)/water-cooledElectric heater band or PeltierElectric heater band
1425 mL minimal working volume when used with vessel stand and heating blanket
2C-Flex is for optical pH version only.
3PC = Polycarbonate, PS = Polystyrene