BioFlo® 320 Vessels

BioFlo® 320 Vessels
BioFlo® 320 Vessels
BioFlo® 320 Vessels
BioFlo® 320 Vessels
BioFlo® 320 Vessels
BioFlo® 320 Vessels
play BioFlo® 320 Vessels

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Product Information

BioFlo 320 vessels feature an autoclavable borosilicate glass body and stainless steel head plate. Stainless-steel dished-bottom vessels are available for rapid heat transfer as well as traditional water-jacketed vessels for more gentle temperature control. Each vessel type is available in four sizes, with interchangeable overhead magnetic and direct drive options. A variety of impellers and multiple industry standard head plate ports are available for user flexibility.

Features

  • Working volume range between 0.6 – 10.5 L
  • Stainless steel dish bottom and water jacketed vessels
  • Interchangeable magnetic and direct overhead drive options
  • Rushton, pitched-blade, marine, spinfilter, cell-lift and packed-bed impellers available

Applications

  • Research and Development in cell culture and microbiology
  • Laboratory and pilot scale fermentation of aerobic and anaerobic bacteria, yeasts, and fungi
  • Cultivation of mammalian, insect, and human cell lines
  • Specialized applications such as stem cell culture or biofuel/biopolymer development
  • Specialized packed-bed impeller for vaccine production in anchorage and non-anchorage dependent cell lines
  • Suitable for batch, fed-batch, and continuous processes
  • Validation packages available for GMP-regulated processes
 1 L3 L5 L10 L
Bacteria/Yeast/Fungiyesyesyesyesyes
Insectyesyesyesyesyes
Mammalian/Animalyesyesyesyesyes
MaterialBorosilicate glass, 316 L stainless steel, EPDM o-ringsBorosilicate glass, 316 L stainless steel, EPDM o-ringsBorosilicate glass, 316 L stainless steel, EPDM o-ringsBorosilicate glass, 316 L stainless steel, EPDM o-rings
Plant/Algaeyesyesyesyesyes
Stem cellsyesyesyesyesyes
Standard set-upBenchtop (BioFlo/CelliGen)
Recommended sensor lengths     
CO2220 mm220 mm320 mm420 mm
DO220 mm220 mm320 mm420 mm
DO (packed-bed)120 mm120 mm220 mm220 mm
Redox200 mm200 mm200 mm425 mm
pH200 mm225 mm325 mm425 mm
pH (packed-bed)200 mm225 mm325 mm225 mm
Stainless Steel Dished Bottom Vessels     
Height20.4 in22.8 in24.1 in26.7 in
Outer diameter (O.D.)7.8 in9.0 in10.1 in11.5 in
Water-Jacketed Vessels     
Height21.8 in24.4 in25.7 in28.7 in
Outer diameter (O.D.)8.5 in9.1 in10.9 in12.7 in
Gassing     
Gas SupplyOverlay and/or SpargerOverlay and/or SpargerOverlay and/or SpargerOverlay and/or Sparger
Sparger TypeRingsparger or microspargerRingsparger or microspargerRingsparger or microspargerRingsparger or microsparger
Bioreactors     
Working volume (total)0.6 – 1.9 L (2.5 L)1.3 – 3.8 L (5 L)1.9 – 5.6 L (7.5 L)3.5 – 10.5 L (14 L)
Agitation     
DriveDirect/magnetic overhead driveDirect/magnetic overhead driveDirect/magnetic overhead driveDirect/magnetic overhead drive
Speed ranges25 – 1,200 rpm (direct drive)
25 – 500 rpm (magnetic drive)
25 – 1,200 rpm (direct drive)
25 – 500 rpm (magnetic drive)
25 – 1,200 rpm (direct drive)
25 – 500 rpm (magnetic drive)
25 – 1,200 rpm (direct drive)
25 – 500 rpm (magnetic drive)
Impellers2 x Rushton, 1 x Pitched, 1x Marine, Spin Filter, Cell Lift, or Packed-bed2 x Rushton, 1 x Pitched, 1x Marine, Spin Filter, Cell Lift, or Packed-bed2 x Rushton, 1 x Pitched, 1x Marine, Spin Filter, Cell Lift, or Packed-bed2 x Rushton, 1 x Pitched, 1x Marine, Spin Filter, Cell Lift, or Packed-bed
Monitoring and Control     
Temperature sensorPt100 RTDPt100 RTDPt100 RTDPt100 RTD
Exhaust condensationWater-cooledWater-cooledWater-cooledWater-cooled