Eppendorf BioSpectrometer® basic

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Eppendorf BioSpectrometer® basic

You need a scan?
The Eppendorf BioSpectrometer® basic makes your work easier: convenient handling, guided software procedures, operation directly on the device, preprogrammed methods, automatic evaluation of measurement data, and clear presentation of results. More information

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Product Information

Building on the legacy of the Eppendorf BioPhotometer D30, the Eppendorf BioSpectrometer basic shares its simplicity, flexibility and reliability. In addition to the Biophotometer D30, the Biospectrometer basic has freely selectable wavelengths from 200 – 830 nm (selectable in 1 nm increments). This allows the user to define the parameters of each assay, whether it be a scan, a single-wavelength measurement or a multi-wavelength measurement. The Eppendorf BioSpectrometer basic caters to more disciplines as the wavelengths are not pre-defined and allows for non-routine assays or new assays to be easily programmed (in addition to the pre-programmed routine methods). The Eppendorf BioSpectrometer basic is a robust yet flexible ˝workhorse˝.

Looking for the target?


The Eppendorf µCuvette is optimized for very low sample volumes. The hydrophobic surface coating on quartz glas enables convenient sample loading.

Eppendorf BioSpectrometer® basic

Left? Right? Up? Down?


Clear indications for positioning the cuvette for convenient handling and safe results.

Eppendorf BioSpectrometer® basic

Believing in raw data?


In general, the purity of a DNA solution is easily defined using purity ratios. It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity.
For this reason, the BioSpectrometer is capable of recording a “Purity Scan” which displays the absorbance properties of a sample graphically, thus enabling fast and simple visual quality control.

Eppendorf BioSpectrometer® basic

Looking for easy-to-read results?


In addition, all important raw data pertaining to the DNA sample are displayed in table format. The table optionally lists the purity ratios (A260/A230 and A260/A280), the absorbance values (raw data), as well as the background absorbance, e.g. at 320 nm.
Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample.

Eppendorf BioSpectrometer® basic

Annoyed by uncomfortable handling?


Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
› Guided software for easy handling
› Clear indications at cuvette shaft for convenient handling
› Direct keys for convenient handling
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Eppendorf BioSpectrometer® basic

Struggling with paper-based documentation?


The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Eppendorf BioSpectrometer® basic

Features

  • Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm)
  • Automatic evaluation and storage of measurement data with clear presentation of results
  • Spectral graph to display sample purity with automatic ratio calculation
  • Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters
  • Two-wavelength method with substraction or division evaluation
  • Integrated application and results memory
  • Guided software process to minimize errors
  • Integrated self-test and calibration history
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email

Applications

  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
  • Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytoxicity endpoint assay
  • Transmission measurement
Eppendorf BioSpectrometer® basic
Video: Eppendorf BioSpectrometer® basic – Video – University of East London

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