Eppendorf BioSpectrometer® fluorescence

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Eppendorf BioSpectrometer® fluorescence
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Eppendorf BioSpectrometer® fluorescence
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Eppendorf BioSpectrometer® fluorescence
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Eppendorf BioSpectrometer® fluorescence
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Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Promotion
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence
play Eppendorf BioSpectrometer® fluorescence
. Eppendorf BioSpectrometer® fluorescence

The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. A variety of cuvettes expands the options even further from microvolume to macrovolume measurements. More information

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Product Information

The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. UV/Vis assays, fluorescence assays and semi-micro/micro-volume measurements can all be carried out on a single instrument without the need for external control (PC/Laptop). Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. Pre-programmed (modified) methods for Qubit DNA quantification kits (SS, HS, BR) expand the flexibility and functionality of the instrument. Additionally, the use of the Eppendorf µCuvette G1.0 for fluorescence assays (5 µL loading volume) allows for a vast reduction in reagent usage.

Features

  • All Eppendorf BioSpectrometer basic options
  • Preprogrammed and freely programmable fluorescence methods
  • Preprogrammed (modified) methods for Qubit DNA quantification kits (SS, HS, BR)
  • Fluorescence measurement concentration of dsDNA down to 1 pg/µL, using Pico Green®
  • Fluorescence measurement concentration of BSA down to 0.01 ng/µL
  • Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
  • Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein
  • Single consumable (UVette) for UV/Vis and fluorescence assays

Applications

  • Ideal for NGS applications (micro-volume UV/Vis and fluorescence assays in a single instrument)
  • dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
  • ssDNA quantification down to 10 pg/µL
  • RNA quantification down to 20 pg/µL
  • Oligo quantification down to 10 pg/µL
  • Protein (BSA) quantification down to 0.01 ng/µL
  • Quantification of GFP-binding proteins
  • Growth measurement of GFP-binding bacteria
 Eppendorf BioSpectrometer® fluorescenceEppendorf BioSpectrometer® fluorescence
Absorption measuring range0 A – 3.0 A (260 nm)
BSA concentration range (UV 280 nm) µCuvette G1.0758 ng/µL - 45,450 ng/µL
±30 ng/µL at A = 0
±76 ng/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm75 ng/µL - 4,545 ng/µL
±3 ng/µL at A = 0
±7 ng/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm379 ng/µL - 22,725 ng/µL
±15 ng/µL at A = 0
±38 ng/µL at A = 1 (0.5 %)
BSA concentration range (for 1 to 10 mm path length)0.01 ng/µL – 45,450 ng/µL
(The factor for BSA is 1,515)
Beam receiver fluorescencePhotodiodes
Cuvette shaft12.5 mm × 12.5 mm
Detector TypeCMOS photodiode array, 1024 pixel
Emission wavelength I fluorescence520 nm, bandwidth: 15 nm
Emission wavelength II fluorescence560 nm, bandwidth: 40 nm
Excitation wavelength fluorescence470 nm, bandwidth: 25 nm
Fluorescence measuring range0.5 nM – 2,000 nM fluorescein (emission wavelength 520 nm)
Light source absorptionXenon flash lamp
Light source fluorescenceLED
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescenceConfocal filter fluorometer with reference beam
Method memory>100 method programs
Mode absorptionSpectrophotometer
Mode fluorescenceFluorometer
Operator guidance languageSpanish, Italian, French, English, German, Japanese
Power consumptionApprox. 15 W during operating step
Approx. 5 W with dimmed display
Random error absorption≤0.002 at A = 0
≤0.005 (0.5 %) at A = 1
Random error fluorescence±2 % at 1 nM fluorescein
Random error wavelength±1 nm
Random temperature error
Sample light path length8.5 mm
Scanningyes
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption
Systematic error wavelength≤0.5 nm
Systematic temperature error
Wavelength Selection Increment1 nm
Wavelength range200 nm – 830 nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range (UV 260 nm) UVette 10 mm2.5 ng/µL – 150 ng/µL
dsDNA concentration range (UV 260 nm) UVette 2 mm12.5 ng/µL – 750 ng/µL
dsDNA concentration range (UV 260 nm) µCuvette G1.025 ng/µL – 1,500 ng/µL
dsDNA concentration range (for 1 to 10 mm path length)1.0 pg/µL – 1,500 ng/µL
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC (All functions available without PC)
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument
Power supply100 – 120 V, 50 – 60 Hz120 V
Dimensions (W × D × H)295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
Weight w/o accessories5.4 kg
Interval between two measuring points
Measuring time range
Adjustable temperature range

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