BioBLU® c Single-Use Vessels

Made from monolayer virgin plastics the Eppendorf BioBLU Single–Use Vessels address the widely discussed problems associated with leachables and extractables (L&E), helping to make laboratories more efficient and safe. The rigid wall stirred–tank design mimicks traditional vessels and makes scale-up easy.
Working Volume Range
Description Catalog No. Net Price Your Price Quantity
BioBLU® 1c Single-Use Vessel, cell culture, 2 pitched-blade impellers, sterile, 4-pack Details 78903506   Inquire Add to favorites
BioBLU® 0.3c Single-Use Vessel, cell culture, 1 pitched-blade impeller, sterile, 4-pack Details 78903508   Inquire Add to favorites
BioBLU® 1c Single-Use Vessel, cell culture, 2 pitched-blade impellers, optical pH, sterile, 4-pack Details 78903510   Inquire Add to favorites
BioBLU® 1c Single-Use Vessel, cell culture, 1 pitched-blade impeller, sterile, 4-pack Details 78903511   Inquire Add to favorites
BioBLU® 1c Single-Use Vessel, cell culture, 1 pitched-blade impeller, optical pH, sterile, 4-pack Details 78903512   Inquire Add to favorites
BioBLU® 3c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 1-pack Details 1386000100   Inquire Add to favorites
BioBLU® 3c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 4-pack Details 1386000200   Inquire Add to favorites
BioBLU® 3c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 1-pack Details 1386000300   Inquire Add to favorites
BioBLU® 3c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 4-pack Details 1386000400   Inquire Add to favorites
BioBLU® 5p Single-Use Vessel, cell culture, microsparger, packed-bed impeller, sterile, 1-pack Details M1363-0119   Inquire Add to favorites
BioBLU® 5p Single-Use Vessel, cell culture, microsparger, packed-bed impeller, sterile, 4-pack Details M1363-0120   Inquire Add to favorites
BioBLU® 5c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 1-pack Details M1363-0121   Inquire Add to favorites
BioBLU® 14c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 1-pack Details M1363-0122   Inquire Add to favorites
BioBLU® 5c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 4-pack Details M1363-0123   Inquire Add to favorites
BioBLU® 14c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, sterile, 4-pack Details M1363-0124   Inquire Add to favorites
BioBLU® 5c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 1-pack Details M1363-0125   Inquire Add to favorites
BioBLU® 14c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 1-pack Details M1363-0126   Inquire Add to favorites
BioBLU® 5c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 4-pack Details M1363-0127   Inquire Add to favorites
BioBLU® 14c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, sterile, 4-pack Details M1363-0128   Inquire Add to favorites
BioBLU® 50c Single-Use Vessel, cell culture, macrosparger, 1 pitched blade impeller, sterile, 1-pack Details M1363-0129   Inquire Add to favorites
BioBLU® 50c Single-Use Vessel, cell culture, macrosparger, 1 pitched blade impeller, sterile, 4-pack Details M1363-0130   Inquire Add to favorites
BioBLU® 50c Single-Use Vessel, cell culture, microsparger, 1 pitched blade impeller, sterile, 1-pack Details M1363-0131   Inquire Add to favorites
BioBLU® 50c Single-Use Vessel, cell culture, microsparger, 1 pitched blade impeller, sterile, 4-pack Details M1363-0132   Inquire Add to favorites
BioBLU® 5p Single-Use Vessel, cell culture, macrosparger, packed-bed impeller, sterile, 1-pack Details M1363-0133   Inquire Add to favorites
BioBLU® 5p Single-Use Vessel, cell culture, macrosparger, packed-bed impeller, sterile, 4-pack Details M1363-0134   Inquire Add to favorites

Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing. BioBLU c and p vessels cover a wide variety of processes, including shear-sensitive suspension cell cultures and adherent cell line development.

Features

  • Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 100 mL to 40 L working volume
  • All BioBLU c vessel sizes available with pitched-blade impellers for cell culture applications
  • BioBLU 5p vessels with Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel® Disks
  • Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
  • Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
  • Raw materials used to produce BioBLU vessels comply with USP Class VI standards and are BSE/TSE free
  • Vessels are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
  • For use with Eppendorf benchtop bioreactor systems
  • Adaptor kits for legacy and third-party bioreactor controllers available
  • Validation packages available upon request

Applications

  • Insect, mammalian, and human suspension cell lines
  • Stem cells
  • Adherent cells
 
 BioBLU® 1cBioBLU® 0.3cBioBLU® 3cBioBLU® 5pBioBLU® 14cBioBLU® 50c
Insectyesyesyesyesyesyes
Mammalian/Animalyesyesyesyesyesyes
Stem cellsyesyesyesyesyesyes
Validatableyesyesyesyesyesyes
ApplicationCell culture2Cell culture2Cell culture2Cell culture2Cell culture2Cell culture2
Standard set-upDASGIP BioblockDASboxBioblock/Benchtop/Third-partyBioblock/Benchtop/Third-partyBioblock/Benchtop/Third-partyBioblock/Benchtop/Third-party
Bioreactors      
SterilizationPre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)
Working volume (total)320 mL – 1.25 L (1.5 L)1100 – 250 mL (380 mL)1.25 – 3.75 L (5 L)3.75 L (5 L)3.5 – 10.5 L (14 L)18 – 40 L (50 L)
Material vesselPS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)
Material TubingSiliconeSiliconeSilicone, C-FlexSilicone, C-FlexSilicone, C-FlexSilicone, C-Flex
Head plate ports3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 1x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harbest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust
Agitation      
DriveMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drives
ImpellersPitched-bladePitched-bladePitched-bladePacked-bedPitched-bladePitched-blade
Recommended agitation speed30 – 600 rpm20 – 500 rpm25 – 200 rpm25 – 200 rpm25 – 200 rpm25 – 150 rpm
Gassing      
Gas supplySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlaySparger and/or overlay
Monitoring and Control      
Sensor length220 mm120 mm225 mm
T sensorPt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)
Max. operating temperature40 °C40 °C40 °C40 °C40 °C40 °C
pH sensorOptical pH or standard glass sensor (220 mm)Optical pH or standard glass sensor (120 mm)Optical pH or standard glass sensorOptical pH or standard glass sensorOptical pH or standard glass sensorOptical pH or standard glass sensor
DO SensorPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/118 mmPolarographic DO - 12/225 mmPolarographic DO - 12/120 mmPolarographic DO - 12/355 mmPolarographic DO - 12/526 mm
Exhaust condensationLiquid-free cooling (Peltier)Liquid-free cooling (Peltier)Electric heater band or PeltierElectric heater band or PeltierElectric heater bandElectric heater band
1425 mL minimal working volume when used with vessel stand and heating blanket

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