BioBLU® f Single-Use Vessels

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BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels

BioBLU f Single-Use Vessels are specifically designed for robust microbial applications using bacteria, yeasts, and fungi allowing microbial process development at the highest level. The rigid wall stirred tank design mimicks traditional vessels and supports effective mixing and mass transfer making them suitable for high-cell density fermentation. More information

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Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate labor-intensive cleaning, improve turn-around time, simplify validation, and reduce overall costs. BioBLU f vessels are specifically designed for robust microbial applications using bacteria, yeasts, and fungi.

Features

  • Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 3.75 L working volume
  • Designed for high-density fermentation processes
  • Multiple Rushton-type impellers for efficient mixing and mass transfer
  • Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
  • Eppendorf exclusive interior cooling baffles for excellent mixing and mass transfer
  • Raw materials used to produce BioBLU vessels comply with USP Class VI standards and are BSE/TSE free
  • Vessels are assembled with sparger, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
  • For use with Eppendorf benchtop bioreactor systems
  • Validation packages available upon request

Applications

  • Bacteria, yeasts, and fungi
  • Suitable for high-cell density fermentation
 BioBLU® 1fBioBLU® 0.3fBioBLU® 3f
Bacteria/Yeast/Fungiyesyesyes
Validatableyesyes
ApplicationMicrobiologyMicrobiologyMicrobiology
Standard set-upBioblock/Benchtop/Third-partyDASboxBioblock/Benchtop/Third-party
Bioreactors   
SterilizationPre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)
Working volume (total)250 mL – 1.25 L (1.5 L)65 – 250 mL (380 mL)1.25 L – 3.75  L (5 L)
Material vesselPS, PC (USP Class VI)PS, PC (USP Class VI)PC (USP Class VI)
Material TubingSiliconeSiliconeSilicone
Head plate ports3x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles (incl. cooling water connection)2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust4x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles (incl. cooling water connection)
Agitation   
DriveMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drives
ImpellersRushton-typeRushton-typeRushton-type
Recommended agitation speed100 – 1,500 rpm20 – 2,000 rpm25 – 1,200 rpm
Gassing   
Gas supplySpargerSpargerSparger
Monitoring and Control   
Sensor length220 mm120 mm
T sensorPt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)
Max. operating temperature45 °C45 °C45 °C
pH sensorStandard glass sensor (220 mm)Standard glass sensor (120 mm)Standard glass sensor
DO SensorPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/118 mmPolarographic DO - 4.7/220 mm
Exhaust condensationLiquid-free cooling (Peltier)Liquid-free cooling (Peltier)Water-cooled